When Mary Queen of Scots dies, she is lying in bed with her eyes closed. Her nephew leans over to give her a kiss and they soom in just as she opens her eyes.
Okay, first off, it was Mary of Guise. Second, it was the other way around. Her eyes were open, and as Norfolk moved his hand to her face, her eyes closed.
The "Mary" that was assassinated in this scene of the movie was the Mary of Scots. Remember, it is only a movie. In real life, Mary of Scots was sentenced to death, thus beheaded. Mary of Scots was known historically as the person who tried to take the life of Queen Elizabeth. Mary TUDOR was the one who died in the beginning of the film from sickness, the queen who is the daughter of Katherine, one of Henry VIII's ex-wives. Mary of Guise IS the mother of Mary of Scots, but she didn't have a relevant role in this time of the movie with Queen Elizabeth, or not as much as Mary of Scots did. I would just go with the idea that the film made a mistake, or just created their own part of the story by allowing Mary of Scots to die of assassination and not of a death sentence.
The "Mary" that was killed in this particular scene is Mary of Guise NOT Mary of Scots. Mary of Scots is the daughter of Mary of Guise. Mary of Scots was convicted of treason against Elizabeth and beheaded, this is true. You actually NEVER see Mary of Scots in this movie. Mary of Scots is the Mary in The Golden Age and she is beheaded in that movie. If you know anything about this era of history then you would know that the name "Mary" is extremely common. Off the top of my head I can think of at least four "Marys" from this particular part of history, so it is easy to get all of them mixed up. "Bloody Mary" dies towards the beginning of the movie so I have no idea how she got thrown into this mess. I hope people actually learned something from this comment...